3K9V
Crystal structure of rat mitochondrial P450 24A1 S57D in complex with CHAPS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-03-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.609, 81.647, 108.699 |
| Unit cell angles | 90.00, 122.89, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.20866 |
| Rwork | 0.206 |
| R-free | 0.25154 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tqn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.136 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.290 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.150 | 0.560 |
| Number of reflections | 58240 | |
| <I/σ(I)> | 15.6 | 4.1 |
| Completeness [%] | 98.8 | 98.2 |
| Redundancy | 10.1 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 280 | 15% PEG 3350, 0.1M Tris-HCl, 0.2 M potassium thiocyanate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 280K |
