3K93

Crystal structure of phage related exonuclease (YP_719632.1) from HAEMOPHILUS SOMNUS 129PT at 2.15 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-08
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97947
Spacegroup name	P 3 2 1
Unit cell lengths	113.369, 113.369, 42.284
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.341 - 2.150
R-factor	0.174
Rwork	0.172
R-free	0.21200
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.459
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.5)
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.341	28.340	2.210
High resolution limit [Å]	2.150	9.620	2.150
Rmerge	0.129	0.055	0.836
Total number of observations		1365	9647
Number of reflections	17252
<I/σ(I)>	12.8	11.5	2.1
Completeness [%]	100.0	96.6	100
Redundancy	7.4	6.4	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	10.0000% Glycerol, 1.0000M NaCl, 30.0000% PEG-600, 0.1M Cacodylate pH 6.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K