3K7Z

GCN4-Leucine zipper core mutant as N16A trigonal automatic solution

Replaces:  1RB1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	90
Detector technology	CCD
Collection date	1997-12-05
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0688, 0.9800, 0.9795, 0.9322
Spacegroup name	P 31 2 1
Unit cell lengths	89.943, 89.943, 46.481
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.960 - 1.900
R-factor	0.204
Rwork	0.202
R-free	0.25900
Structure solution method	MAD
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	ELVES
Refinement software	REFMAC (4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.097
Number of reflections	23195
Completeness [%]	99.6	98.4
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	298	100MM BIS-TRIS buffer only, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 298K