3K7V
Protein phosphatase 2A core complex bound to dinophysistoxin-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0809 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 92.830, 194.780, 202.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.520 - 2.850 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.26200 |
Structure solution method | Rigid body refinement |
Starting model (for MR) | 2ie4 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.850 | 2.850 |
Number of reflections | 42933 | |
Completeness [%] | 99.6 | 99.4 |
Redundancy | 4.5 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M LITHIUM SULFATE, 1.5 M AMMONIUM SULFATE, 0.1 M TRIS-CL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |