3K7U
Structure of essential protein from Trypanosoma brucei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-06-25 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.362, 50.359, 44.150 |
| Unit cell angles | 90.00, 108.97, 90.00 |
Refinement procedure
| Resolution | 29.690 - 2.100 |
| R-factor | 0.20538 |
| Rwork | 0.202 |
| R-free | 0.26459 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k80 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.842 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0095) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.060 |
| High resolution limit [Å] | 2.100 | 1.990 |
| Number of reflections | 15229 | |
| <I/σ(I)> | 30.6 | 3.1 |
| Completeness [%] | 96.4 | 76.2 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 25% PEG2000, 0.1 M Bis-tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
