3K7J
Crystal structure of the D100E mutant of the Indian Hedgehog N-terminal signalling domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-02-08 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.160, 52.400, 34.660 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 18.721 - 1.900 |
| R-factor | 0.2035 |
| Rwork | 0.200 |
| R-free | 0.27570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vhh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.886 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.730 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.079 | 0.079 |
| Number of reflections | 11849 | |
| <I/σ(I)> | 10.7 | 2.4 |
| Completeness [%] | 98.8 | 97.5 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 30% PEG 4000, 0.2M (NH4)2SO4, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
