3K7H
Crystal structure of the E95K mutant of the Indian Hedgehog N-terminal signalling domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.890, 52.750, 35.440 |
| Unit cell angles | 90.00, 90.54, 90.00 |
Refinement procedure
| Resolution | 26.971 - 1.500 |
| R-factor | 0.1902 |
| Rwork | 0.190 |
| R-free | 0.19780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vhh |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.995 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.971 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.048 | 0.656 |
| Number of reflections | 31798 | |
| <I/σ(I)> | 14.7 | 2 |
| Completeness [%] | 99.5 | 98.4 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.1M sodium cacodylate, pH 6.5, 30% PEG 8000, 0.2M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
