3K6S
Structure of integrin alphaXbeta2 ectodomain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97926,0.97942,0.96419 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 132.095, 163.561, 536.911 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.613 - 3.500 |
| R-factor | 0.2975 |
| Rwork | 0.297 |
| R-free | 0.33470 |
| Structure solution method | MAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.648 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.610 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.161 | 1.371 |
| <I/σ(I)> | 12 | 1.93 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.53 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 277 | 0.55M tri-sodium citrate, 0.1M imidazole, pH 6.5, EVAPORATION, temperature 277K |
