3K5W
Crystal structure of a Carbohydrate kinase (YjeF family)from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 119.955, 119.955, 162.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.230 - 2.600 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.26200 |
| Structure solution method | SAD |
| RMSD bond angle | 0.009 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS (& SHARP) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Number of reflections | 25967 | |
| <I/σ(I)> | 10.8 | 7.8 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 28.1 | 27.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 0.1M KH2PO4, 0.2M Ammonium Citrate pH 7.0 8% Acetonitrile 15% PEG 3,350., VAPOR DIFFUSION, SITTING DROP, temperature 292K |
