3K5N
Crystal structure of E.coli Pol II-abasic DNA binary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-12-27 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.412, 147.552, 96.279 |
| Unit cell angles | 90.00, 99.98, 90.00 |
Refinement procedure
| Resolution | 31.310 - 3.150 |
| Rwork | 0.232 |
| R-free | 0.23600 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.327 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.131 | 0.569 |
| Number of reflections | 41921 | |
| <I/σ(I)> | 11 | 2.6 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350, 0.3 M ammonium formate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
