3K5E
The structure of human kinesin-like motor protein Kif11/KSP/Eg5 in complex with ADP and enastrol.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.580, 79.800, 69.190 |
| Unit cell angles | 90.00, 96.93, 90.00 |
Refinement procedure
| Resolution | 39.900 - 1.970 |
| R-factor | 0.24381 |
| Rwork | 0.241 |
| R-free | 0.29489 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q0b |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.337 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.900 | 2.070 |
| High resolution limit [Å] | 1.970 | 2.000 |
| Rmerge | 0.111 | 0.445 |
| Number of reflections | 56420 | |
| <I/σ(I)> | 5.3 | 2.8 |
| Completeness [%] | 93.9 | 98.8 |
| Redundancy | 2.34 | 2.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 294 | 25% PEG3350, 0.1M BisTris pH 6.0, 0.2M Ammonium sulphate, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
