3K4G
Crystal structure of E. coli RNA polymerase alpha subunit C-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-24 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.342, 67.612, 116.553 |
Unit cell angles | 90.00, 90.12, 90.00 |
Refinement procedure
Resolution | 47.023 - 2.050 |
R-factor | 0.194 |
Rwork | 0.193 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lb2 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.775 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | PHASER (1.3.3) |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.036 | 2.160 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.082 | 0.544 |
Total number of observations | 26693 | |
Number of reflections | 50235 | |
<I/σ(I)> | 12.9 | 2.1 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M hepes, 1.4M sodium citrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |