3K46
Crystal structure of full-length E. coli beta-glucuronidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-18 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.745, 76.193, 125.666 |
| Unit cell angles | 90.00, 125.00, 90.00 |
Refinement procedure
| Resolution | 30.620 - 2.500 |
| R-factor | 0.217 |
| Rwork | 0.214 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k4a |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.815 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.497 | 2.500 |
| Rmerge | 0.125 | 0.452 |
| Number of reflections | 43689 | |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 96.5 | 82.4 |
| Redundancy | 5.4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 289 | 15% PEG 3350, 0.2M Mg Acetate, 0.02% Sodium azide, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
