3K44

Crystal Structure of Drosophila melanogaster Pur-alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-04
Detector	ADSC QUANTUM 210
Spacegroup name	P 1 2 1
Unit cell lengths	63.168, 62.544, 64.479
Unit cell angles	90.00, 90.01, 90.00

Refinement procedure

Resolution	44.460 - 2.100
R-factor	0.223
Rwork	0.222
R-free	0.24000
Structure solution method	MAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRUNCH2
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.479	2.230
High resolution limit [Å]	2.100	2.100
Rmerge		0.442
Number of reflections	28601
<I/σ(I)>		4.35
Completeness [%]	98.9	94.6
Redundancy		5.65

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50 mM Mes, 200 mM MgCl2, 25% PEG 3350, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP		298	50 mM Mes, 200 mM MgCl2, 25% PEG 3350, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K