3K42
Crystal structure of sCD-MPR mutant E19Q/K137M pH 7.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 98 |
Detector technology | IMAGE PLATE |
Collection date | 2009-04-14 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | I 4 |
Unit cell lengths | 102.582, 102.582, 100.145 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.270 - 2.300 |
Rwork | 0.228 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m6p |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.093 | 0.481 |
Number of reflections | 23014 | |
<I/σ(I)> | 9.7 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.2 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 0.1M HEPES pH 7.0, 3M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 292K |