3K3V
Crystal structure the GYF domain of S. Cerevisiae SMY2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-19 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.91841 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 68.400, 68.400, 111.321 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.190 - 1.800 |
Rwork | 0.208 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fma |
RMSD bond length | 0.011 |
RMSD bond angle | 1.497 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.190 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.075 | 0.656 |
Total number of observations | 42500 | |
Number of reflections | 14884 | |
<I/σ(I)> | 31.4 | 5.2 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 20.9 | 20.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 18% polyacrylic acid, 0.15M MgCl2, 0.1M Na-HEPES, pH 7.5, Vapor diffusion, sitting drop, temperature 293K |