3K3F

Crystal Structure of the Urea Transporter from Desulfovibrio Vulgaris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.01
Spacegroup name	P 63
Unit cell lengths	110.131, 110.131, 84.861
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.780 - 2.300
R-factor	0.18
Rwork	0.179
R-free	0.20400
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.469
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.089	0.964
Number of reflections	25924
<I/σ(I)>	14.4
Completeness [%]	99.4	95.2
Redundancy	8.2	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	23% PEG 1500, 100 mM Na Cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	23% PEG 1500, 100 mM Na Cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K