3K3B
Co-crystal structure of the human kinesin Eg5 with a novel tetrahydro-beta-carboline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.953724 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.970, 92.670, 102.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.960 - 2.400 |
| R-factor | 0.2075 |
| Rwork | 0.205 |
| R-free | 0.24340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.055 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | EPMR |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.960 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.120 | 0.462 |
| Number of reflections | 29909 | |
| <I/σ(I)> | 6.6 | 2.5 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 15-17% PEG3350 150-300 mM NaNO3 100 mM MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
