3K21
Crystal Structure of carboxy-terminus of PFC0420w.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | .97920 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 50.469, 50.469, 108.656 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 16.730 - 1.150 |
| R-factor | 0.152 |
| Rwork | 0.150 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.404 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.170 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.078 | 0.547 |
| Number of reflections | 56865 | |
| <I/σ(I)> | 11.8 | 1.57 |
| Completeness [%] | 98.2 | 77.6 |
| Redundancy | 9 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 22 % PEG 3350, 0.1 M NaAcet pH 4.2, 10 mM CaCl2, 2 mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
