3K1L
Crystal Structure of FANCL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0397 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 188.680, 188.680, 259.360 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 77.926 - 3.200 |
| R-factor | 0.2072 |
| Rwork | 0.205 |
| R-free | 0.24660 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.126 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.926 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.089 | 0.479 |
| Number of reflections | 25772 | |
| <I/σ(I)> | 6.3 | 5.2 |
| Completeness [%] | 98.8 | 100 |
| Redundancy | 12.2 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 1M Ammonium Citrate, 100mM Bis-Tris-Propane, 1mM TCEP, 0.050mM ZNCl2, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
