3K1D

Crystal structure of glycogen branching enzyme synonym: 1,4-alpha-D-glucan:1,4-alpha-D-GLUCAN 6-glucosyl-transferase from mycobacterium tuberculosis H37RV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9897
Spacegroup name	P 21 21 2
Unit cell lengths	109.373, 156.861, 48.021
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.970 - 2.330
R-factor	0.193
Rwork	0.193
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB CODE 1M7X
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.410
High resolution limit [Å]	2.330	2.330
Number of reflections	36272
<I/σ(I)>	12.9	2.5
Completeness [%]	99.9	100
Redundancy	14.4	15.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	297	pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 297K