3K0W
Crystal structure of the tandem IG-like C2-type 2 domains of the human mucosa-associated lymphoid tissue lymphoma translocation protein 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97920 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 90.355, 90.355, 182.173 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.470 - 2.800 |
R-factor | 0.2196 |
Rwork | 0.218 |
R-free | 0.24890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bfo |
RMSD bond length | 0.010 |
RMSD bond angle | 1.320 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.470 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 11498 | |
<I/σ(I)> | 43.44 | 2.82 |
Completeness [%] | 99.9 | 91.8 |
Redundancy | 18.8 | 17.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 289 | 1:1 VOLUME RATIO OF PROTEIN SOLUTION (10 mg/mL) AND RESERVOIR SOLUTION (1.5 M MAGNESIUM CHLORIDE, 0.1 M BICINE pH 9.0), VAPOR DIFFUSION, SITTING DROP, temperature 289K |