3JZG

Structure of EED in complex with H3K27me3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ DW
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-08-01
Detector	RIGAKU RAXIS
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	57.775, 85.263, 91.169
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.270 - 2.100
R-factor	0.16107
Rwork	0.158
R-free	0.22177
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	1.931
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall
Low resolution limit [Å]	62.300
High resolution limit [Å]	2.100
Rmerge	0.144
Number of reflections	26778
<I/σ(I)>	5.1
Completeness [%]	99.0
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	300	3.5M NaF, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 300K