3JZA

Crystal structure of human Rab1b in complex with the GEF domain of DrrA/SidM from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-28
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000000
Spacegroup name	P 63 2 2
Unit cell lengths	100.756, 100.756, 146.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.630 - 1.800
R-factor	0.202
Rwork	0.201
R-free	0.23400
Structure solution method	SAD
RMSD bond length	0.024
RMSD bond angle	1.829
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	REFMAC (5.5.0070)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.900
High resolution limit [Å]	1.800	25.000	1.800
Rmerge	0.090	0.029	0.456
Number of reflections	40529	9	5873
<I/σ(I)>	21.97	52.2	4.7
Completeness [%]	98.1	100	97.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.75	292	0.1 M Sodium citrate, 35% v/v PEG 600, pH 5.75, VAPOR DIFFUSION, HANGING DROP, temperature 292K