3JZA
Crystal structure of human Rab1b in complex with the GEF domain of DrrA/SidM from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000000 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 100.756, 100.756, 146.360 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.630 - 1.800 |
| R-factor | 0.202 |
| Rwork | 0.201 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.829 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 25.000 | 1.800 |
| Rmerge | 0.090 | 0.029 | 0.456 |
| Number of reflections | 40529 | 9 | 5873 |
| <I/σ(I)> | 21.97 | 52.2 | 4.7 |
| Completeness [%] | 98.1 | 100 | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.75 | 292 | 0.1 M Sodium citrate, 35% v/v PEG 600, pH 5.75, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
