3JZ4
Crystal structure of E. coli NADP dependent enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-15 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.95364 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 151.885, 151.885, 165.772 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.560 - 2.300 |
R-factor | 0.17 |
Rwork | 0.168 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.297 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.560 | |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.188 | 0.188 |
Number of reflections | 74567 | |
<I/σ(I)> | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.2 M ammonium tartrate, 30 % PEG 3350, 10 mM 2-mercaptoethanol, 0.1 M tris, pH 7.5, temperature 298K, VAPOR DIFFUSION, HANGING DROP |