3JXI

Crystal structure of the chicken TRPV4 ankyrin repeat domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2007-08-08
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	105.259, 48.119, 133.891
Unit cell angles	90.00, 101.89, 90.00

Refinement procedure

Resolution	29.510 - 2.300
R-factor	0.204
Rwork	0.203
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2etb (TRPV2 ankyrin repeats)
RMSD bond length	0.014
RMSD bond angle	1.473
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.4.0066)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	2.280
High resolution limit [Å]	2.200	4.740	2.200
Rmerge	0.078	0.034	0.598
Number of reflections	65393
<I/σ(I)>	9.7
Completeness [%]	97.6	97.3	97.1
Redundancy	2.8	2.9	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	0.1 M sodium citrate pH 5.0, 10% MPD, 2% PEG8000, 4.3% trifluoroethanol, VAPOR DIFFUSION, HANGING DROP, temperature 277K