3JWD

Structure of HIV-1 gp120 with gp41-Interactive Region: Layered Architecture and Basis of Conformational Mobility

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-02-02
Detector	MAR scanner 300 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	83.112, 172.954, 193.144
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.860 - 2.610
Rwork	0.201
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1RZJ 1rz7
RMSD bond length	0.000
RMSD bond angle	0.350
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	PHENIX ((CCI APPS 2007_04_06_1210))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.610	2.610
Rmerge	0.107	0.579
Number of reflections	59657
<I/σ(I)>	20.5	1.38
Completeness [%]	69.7	17.2
Redundancy	5.6	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9.5	293	4.5 % PEG 8000, 01M CHES, pH 9.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K