3JW8
Crystal structure of human mono-glyceride lipase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9797, 0.9799, 1.000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 86.588, 126.311, 140.567 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.210 - 2.100 |
| Rwork | 0.157 |
| R-free | 0.18910 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.320 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.9.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.900 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 44315 | |
| <I/σ(I)> | 18.4 | 6.5 |
| Completeness [%] | 97.8 | 97.4 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 50mM MES, 40% MPD, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
