3JVO

Crystal structure of bacteriophage HK97 gp6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-04
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97931
Spacegroup name	P 21 21 2
Unit cell lengths	196.751, 200.211, 51.169
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.780 - 2.100
R-factor	0.196
Rwork	0.194
R-free	0.22600
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.228
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.074	0.055	0.292
Number of reflections	115130
<I/σ(I)>	11.9
Completeness [%]	97.3	99.3	87.4
Redundancy	5.8	5.6	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	20% MPD, 0.1M calcium chloride, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K