3JVM
Crystal structure of bromodomain 2 of mouse Brd4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97886 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 52.100, 73.150, 32.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.530 - 1.200 |
| R-factor | 0.117 |
| Rwork | 0.116 |
| R-free | 0.15100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ouo |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.366 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.530 | 1.250 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.066 | 0.577 |
| Number of reflections | 37336 | 4265 |
| <I/σ(I)> | 23.39 | 6.9 |
| Completeness [%] | 95.2 | 96.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris/HCl, 25% PEG 2000 MME, soaked with 10 mM H3-K(ac)14 octapeptide GGK(ac)APRKQ, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
