3JV4
Crystal structure of the dimerization domains p50 and RelB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 105 |
Detector technology | IMAGE PLATE |
Collection date | 2007-03-05 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 106.173, 106.173, 143.778 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.760 - 3.150 |
Rwork | 0.225 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.260 |
High resolution limit [Å] | 3.150 | 3.150 |
Number of reflections | 13637 | |
<I/σ(I)> | 8.4 | 1.5 |
Completeness [%] | 82.0 | 77 |
Redundancy | 9 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | PEG8000, ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |