3JUX
Structure of the translocation ATPase SecA from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.29 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.533, 119.403, 120.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.100 |
| Rwork | 0.227 |
| R-free | 0.30300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tf5 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.220 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 17570 | |
| <I/σ(I)> | 13.6 | 2.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.8 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M Hepes pH 7.5, 60% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
