3JUI
Crystal Structure of the C-terminal Domain of Human Translation Initiation Factor eIF2B epsilon Subunit
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9796, 0.9794, 0.9600 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 46.537, 66.082, 136.128 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.160 - 2.000 |
| R-factor | 0.214 |
| Rwork | 0.211 |
| R-free | 0.27900 |
| Structure solution method | MAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.761 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.10) |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.070 | 0.048 | 0.313 |
| Number of reflections | 13260 | ||
| <I/σ(I)> | 13.2 | ||
| Completeness [%] | 90.6 | 99.3 | 84.1 |
| Redundancy | 7.2 | 8.7 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.7 | 289 | PEG8000, Calcium acetate, Sodium Cocadylate, pH 7.7, vapor diffusion, temperature 289K |
