3JUE
Crystal Structure of ArfGAP and ANK repeat domain of ACAP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-10 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.28 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 107.809, 163.473, 41.092 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.300 |
| R-factor | 0.20321 |
| Rwork | 0.202 |
| R-free | 0.21936 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.272 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.630 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.067 | 0.351 |
| Number of reflections | 36997 | |
| <I/σ(I)> | 19.9 | 1.58 |
| Completeness [%] | 97.3 | 81.8 |
| Redundancy | 5.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 289 | 0.2M ammonium sulfate, 14% PEG 3350, 0.1M Sodium Citrate, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
