3JUA

Structural basis of YAP recognition by TEAD4 in the Hippo pathway

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Detector technology	CCD
Collection date	2008-11-13
Detector	ADSC QUANTUM 1
Spacegroup name	P 21 21 21
Unit cell lengths	100.982, 146.910, 165.471
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.000
R-factor	0.23893
Rwork	0.236
R-free	0.28842
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.876
Data scaling software	SCALA (3.2.25)
Refinement software	REFMAC (5.4.0077)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	74.346	74.350	2.900
High resolution limit [Å]	2.800	10.840	2.800
Rmerge	0.060	0.031	0.605
Total number of observations		3535	21139
Number of reflections	61276
<I/σ(I)>	14.2	14.9	1.2
Completeness [%]	99.9	97.8	100
Redundancy	3.6	3.1	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	HANGING DROP	5.6	298	PEG10000, Mg Acetate, pH5.6, HANGING DROP, temperature 298K