3JU1
Crystal Structure of Enoyl-CoA Hydratase/Isomerase Family Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-28 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 83.982, 83.982, 277.832 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.058 - 2.301 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.23100 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.652 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.4_147)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.060 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 50834 | |
| <I/σ(I)> | 13.6 | 5 |
| Completeness [%] | 98.3 | 100 |
| Redundancy | 11.8 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.1M sodium acetate pH 4.6, 2 M sodium formate, 1/10 V8 protease, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
