3JU0
Structure of the arm-type binding domain of HAI7 integrase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0015 |
Spacegroup name | P 65 |
Unit cell lengths | 52.530, 52.530, 79.470 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 6.000 - 1.600 |
R-factor | 0.15065 |
Rwork | 0.148 |
R-free | 0.21211 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3jtz |
RMSD bond length | 0.025 |
RMSD bond angle | 2.051 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 6.000 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.027 | 0.107 |
Number of reflections | 15089 | |
<I/σ(I)> | 30.67 | 9.31 |
Completeness [%] | 91.6 | 76 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 277 | 0.1M MES, pH6.3, 2.8M ammonium sulphate, VAPOR DIFFUSION, SITTING DROP, temperature 277K |