3JSY
N-terminal fragment of ribosomal protein L10 from Methanococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-5 |
| Synchrotron site | MAX II |
| Beamline | I911-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.90778 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.000, 70.080, 97.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.600 |
| R-factor | 0.18412 |
| Rwork | 0.182 |
| R-free | 0.23321 |
| Structure solution method | Solved with MAD and refined against native data set |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.929 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.056 | 0.380 |
| Number of reflections | 50801 | |
| <I/σ(I)> | 21.61 | 4.45 |
| Completeness [%] | 92.5 | 85 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | KSCN, PEG4000, NaAcetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
