3JSO
Classic Protein With a New Twist: crystal structure of a LexA repressor DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-05 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 100.975, 106.935, 65.895 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.530 - 2.290 |
| R-factor | 0.268 |
| Rwork | 0.266 |
| R-free | 0.30600 |
| Structure solution method | MAD, MR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.915 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.290 |
| Number of reflections | 23570 |
| <I/σ(I)> | 21.4 |
| Completeness [%] | 72.2 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 6.8 | 292 | 0.1M Bis-tris propane, 0.05M sodium tartrate, 10% glycerol, 15% ethylene glycol, 14% (w/v) PEG3350, pH 6.8, hanging drop, temperature 292K |
| 1 | hanging drop | 6.8 | 292 | 0.1M Bis-tris propane, 0.05M sodium tartrate, 10% glycerol, 15% ethylene glycol, 14% (w/v) PEG3350, pH 6.8, hanging drop, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Bis-tris propane | ||
| 10 | 1 | 2 | PEG3350 | ||
| 2 | 1 | 1 | sodium tartrate | ||
| 3 | 1 | 1 | glycerol | ||
| 4 | 1 | 1 | ethylene glycol | ||
| 5 | 1 | 1 | PEG3350 | ||
| 6 | 1 | 2 | Bis-tris propane | ||
| 7 | 1 | 2 | sodium tartrate | ||
| 8 | 1 | 2 | glycerol | ||
| 9 | 1 | 2 | ethylene glycol |
