3JSE

Crystal structure of archaeal 20S proteasome in complex with mutated P26 activator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-03
Detector	ADSC QUANTUM 315
Spacegroup name	C 1 2 1
Unit cell lengths	254.701, 126.099, 180.393
Unit cell angles	90.00, 92.00, 90.00

Refinement procedure

Resolution	19.860 - 2.900
R-factor	0.21271
Rwork	0.212
R-free	0.24126
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yar
RMSD bond length	0.017
RMSD bond angle	1.455
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	20.000	3.000
High resolution limit [Å]	2.900	6.190	2.900
Rmerge	0.117	0.054	0.517
<I/σ(I)>	9.6
Completeness [%]	92.9	98.7	82.2
Redundancy	2.9	2.9	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	294	pH 5.6, VAPOR DIFFUSION, PEG 1000, HANGING DROP, temperature 294K, Sodium Phosphate Citrate, LiSo4, Imidazole, VAPOR DIFFUSION, HANGING DROP