3JRV
Structure of poxvirus K7 protein in complex with RNA helicase DDX3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.889, 69.529, 65.626 |
| Unit cell angles | 90.00, 122.17, 90.00 |
Refinement procedure
| Resolution | 55.550 - 1.600 |
| R-factor | 0.143 |
| Rwork | 0.141 |
| R-free | 0.19000 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.490 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.600 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.081 | 0.373 |
| Number of reflections | 44187 | |
| <I/σ(I)> | 35.4 | 3.4 |
| Completeness [%] | 97.3 | 84.9 |
| Redundancy | 7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.25 | 291 | 100mM sodium citrate, 15.0% (w/v) PEG 3350, pH 5.25, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
