3JRP

SEC13 with NUP145C (AA109-179) insertion blade

Experimental procedure

Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	68.280, 93.878, 55.047
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.717 - 2.600
R-factor	0.221
Rwork	0.217
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pm6
RMSD bond length	0.003
RMSD bond angle	0.679
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.660
High resolution limit [Å]	2.600	2.600
Number of reflections	11148
Completeness [%]	97.8	96.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	289	0.1M Tris, 22% PEG 4000, 0.25 M LiCl, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 289K