Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-05 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.227, 75.915, 81.547 |
| Unit cell angles | 90.00, 94.06, 90.00 |
Refinement procedure
| Resolution | 32.440 - 3.000 |
| R-factor | 0.261 |
| Rwork | 0.250 |
| R-free | 0.32000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | T4 lysozyme I3P mutant |
| RMSD bond angle | 0.020 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CCP4 ((refmac)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.440 | |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 17925 | |
| Completeness [%] | 96.5 | 99.8 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% PEG 3400, 50 mM ammonium sulfate, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
