3IXT

Crystal Structure of Motavizumab Fab Bound to Peptide Epitope

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.82656
Spacegroup name	P 43 21 2
Unit cell lengths	90.752, 90.752, 232.057
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.256 - 2.750
R-factor	0.2162
Rwork	0.213
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hwz
RMSD bond length	0.004
RMSD bond angle	0.742
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.750	2.750
Number of reflections	23510
Completeness [%]	93.5	88.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	17.5% PEG 8000, 0.2 M zinc acetate, 0.1 M cacodylate pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K