3IWZ
The c-di-GMP Responsive Global Regulator CLP Links Cell-Cell Signaling to Virulence Gene Expression in Xanthomonas campestris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97966 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.690, 67.690, 110.370 |
Unit cell angles | 90.00, 104.20, 90.00 |
Refinement procedure
Resolution | 29.850 - 2.300 |
R-factor | 0.244 |
Rwork | 0.244 |
R-free | 0.30500 |
Structure solution method | MIR |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.360 |
High resolution limit [Å] | 2.300 | 2.280 |
Rmerge | 0.039 | 0.400 |
Number of reflections | 42637 | |
<I/σ(I)> | 24.5 | 3.1 |
Completeness [%] | 97.1 | 94 |
Redundancy | 4.3 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | Tris 8.5, PEG 3350 20%, VAPOR DIFFUSION, SITTING DROP, temperature 298K |