3IWY
Crystal structure of human MDM2 complexed with D-peptide (12 residues)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-02-24 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.569, 46.503, 49.353 |
| Unit cell angles | 90.00, 92.35, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.930 |
| R-factor | 0.21258 |
| Rwork | 0.210 |
| R-free | 0.25856 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eqs |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.899 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.280 | 1.960 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.117 | 0.471 |
| Number of reflections | 11041 | |
| <I/σ(I)> | 10.8 | 1.9 |
| Completeness [%] | 90.3 | |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 273 | 0.05 M potassium phosphate monobasic, 20% PEG 8,000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
