3IWT
Structure of hypothetical molybdenum cofactor biosynthesis protein B from Sulfolobus tokodaii
Replaces: 2PJKExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.074 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 136.675, 136.675, 210.525 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.740 - 1.900 |
| R-factor | 0.16936 |
| Rwork | 0.168 |
| R-free | 0.18695 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y5e |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.477 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.065 | 0.610 |
| Number of reflections | 91510 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 98.5 | 97.7 |
| Redundancy | 9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 7 | 298 | 10% W/V PEG 8000, 0.1M TRIS-HCL, 0.2M MGCL2, pH 7.0, MICROBATCH UNDER OIL, temperature 298.0K |
