3IWP
Crystal structure of human copper homeostasis protein CutC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 78.160, 78.406, 170.349 |
| Unit cell angles | 76.84, 87.88, 71.86 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.23584 |
| Rwork | 0.233 |
| R-free | 0.28254 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x7i |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.390 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.060 | 0.299 |
| Number of reflections | 127488 | |
| <I/σ(I)> | 23.7 | 4.1 |
| Completeness [%] | 98.5 | 97.9 |
| Redundancy | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 0.1 M citric acid, pH4.0, and 10% polyethylene glycol 6000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
