3IUY

Crystal structure of DDX53 DEAD-box domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Spacegroup name	P 1 21 1
Unit cell lengths	56.442, 61.251, 65.787
Unit cell angles	90.00, 96.36, 90.00

Refinement procedure

Resolution	45.130 - 2.400
R-factor	0.204
Rwork	0.201
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.157
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0035)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.382	45.130	2.530
High resolution limit [Å]	2.400	7.590	2.400
Rmerge	0.187	0.118	0.578
Total number of observations		5056	24409
Number of reflections	17649
<I/σ(I)>	13.4	4.1	1.2
Completeness [%]	100.0	99.6	100
Redundancy	9.3	8.6	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	27% PEG6000, 0.1M MES pH 6, 0.2M AmmoniumChloride, 15% Glycerol, 0.2M NaCl , VAPOR DIFFUSION, SITTING DROP, temperature 277K