3IUG

Crystal structure of the RhoGAP domain of RICS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-06-15
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	102.264, 117.369, 37.717
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.770
R-factor	0.2
Rwork	0.199
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2osa
RMSD bond length	0.018
RMSD bond angle	1.563
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.770	3.810	1.770
Rmerge	0.064	0.032	0.702
Number of reflections	45281
<I/σ(I)>	11
Completeness [%]	99.7	99.6	97.9
Redundancy	7.1	7	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	30% PEG 5000 MME, 0.2M Ammonium sulfate, 0.1M MES, 1:100 w/w subtilisin, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K