3IUG
Crystal structure of the RhoGAP domain of RICS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-06-15 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 102.264, 117.369, 37.717 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.770 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2osa |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.563 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
| High resolution limit [Å] | 1.770 | 3.810 | 1.770 |
| Rmerge | 0.064 | 0.032 | 0.702 |
| Number of reflections | 45281 | ||
| <I/σ(I)> | 11 | ||
| Completeness [%] | 99.7 | 99.6 | 97.9 |
| Redundancy | 7.1 | 7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 30% PEG 5000 MME, 0.2M Ammonium sulfate, 0.1M MES, 1:100 w/w subtilisin, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
